Dermatopathology Laboratory Requisitions, CTA Lab

CTA Lab, Curtis Thompson, MD & Associates Dermatopathology Lab Nail Resources

Periodic Acid-Schiffs Stain (P.A.S.) Stain for Nails

Procedure:

  1. Place a small amount of Gelatin in water bath.
  2. Using positively charged slide, pick up desired sections.
  3. Place in 60 deg. C oven for 30-60 minutes.(More time for “difficult” specimens)
  4. Deparaffinize slides using Xylene or Xylene substitutes and hydrate through alcohols.
  5. Rinse slide in running tap water.
  6. Rinse slide in distilled water.
  7. DO NOT “DIGEST” SLIDES!!!
  8. Place slide in 1% Periodic Acid for 12 minutes.
  9. Rinse slide quickly in distilled water.
  10. Place slide in Schiffs Solution for 15 minutes.
  11. Rinse slides in warm tap water for 5 minutes.
  12. Place slide in Light Green Stain as needed to reach desired background intensity.
    (Approx. 30 seconds)
  13. Dehydrate slide through 3 changes of Absolute Alcohol.
  14. Clear slide through 3 changes of Xylene or Xylene substitute.
  15. Coverslip using permanent mounting media.

Results:
Basement membrane, Fungi, Glycogen and Mucin: PINK TO RED
Other tissue: Green

Reference:
American Master Tech Scientific, Inc. “PAS Kit Procedure.”

Nail Diagram [PDF]




Dermatopathology Laboratory Requisitions, CTA Lab

CTA Lab | PO Box 230577 | Tigard, Oregon 97281-0577
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